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FLI1 expression in HaCaT cells induces a spectrum of genes associated with psoriasis. ( a ) The Kegg pathway enrichment analysis of DEGs identified in HaCaT-FLI1 versus HaCaT-control cells. ( b ) The left panel shows the heatmap of DEGs with significant differences derived from HaCaT-FLI1 versus HaCaT-control cells. The right panel presents the FPKM scores of the indicated genes. ( c ) Relative expression of the indicated genes in the HaCaT-FLI1 and HaCaT-control cells, as determined by RT-qPCR. ( d ) The expression analysis of FLI1 in HaCaT-FLI1 cells treated with either DMSO or 20 nM of <t>Camptothecin</t> <t>(CPT)</t> for 24h, as determined by Western blotting. P ≤0.01 (**), P ≤0.001 (***) and P ≤0.0001 (****).
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FLI1 expression in HaCaT cells induces a spectrum of genes associated with psoriasis. ( a ) The Kegg pathway enrichment analysis of DEGs identified in HaCaT-FLI1 versus HaCaT-control cells. ( b ) The left panel shows the heatmap of DEGs with significant differences derived from HaCaT-FLI1 versus HaCaT-control cells. The right panel presents the FPKM scores of the indicated genes. ( c ) Relative expression of the indicated genes in the HaCaT-FLI1 and HaCaT-control cells, as determined by RT-qPCR. ( d ) The expression analysis of FLI1 in HaCaT-FLI1 cells treated with either DMSO or 20 nM of Camptothecin (CPT) for 24h, as determined by Western blotting. P ≤0.01 (**), P ≤0.001 (***) and P ≤0.0001 (****).

Journal: Journal of Inflammation Research

Article Title: FLI1 Induces Plaque Psoriasis and Its Inhibition Attenuates Disease Progression

doi: 10.2147/JIR.S500822

Figure Lengend Snippet: FLI1 expression in HaCaT cells induces a spectrum of genes associated with psoriasis. ( a ) The Kegg pathway enrichment analysis of DEGs identified in HaCaT-FLI1 versus HaCaT-control cells. ( b ) The left panel shows the heatmap of DEGs with significant differences derived from HaCaT-FLI1 versus HaCaT-control cells. The right panel presents the FPKM scores of the indicated genes. ( c ) Relative expression of the indicated genes in the HaCaT-FLI1 and HaCaT-control cells, as determined by RT-qPCR. ( d ) The expression analysis of FLI1 in HaCaT-FLI1 cells treated with either DMSO or 20 nM of Camptothecin (CPT) for 24h, as determined by Western blotting. P ≤0.01 (**), P ≤0.001 (***) and P ≤0.0001 (****).

Article Snippet: Chelerythrine, IMQ, TPA and Camptothecin (CPT) compounds were obtained from MCE (MCE, CN).

Techniques: Expressing, Control, Derivative Assay, Quantitative RT-PCR, Western Blot